Summers' Lab Protocols
(click protocol to download PDF file)

1. Alkaline lysis plasmid prep.
2. Boiling lysis plasmid prep.
3. Nostoc Plasmid prep.
4. Triparental conjugation to Nostoc
5. Chlorophyll extraction
6. Colony PCR
7. Electroporation of Nostoc punctiforme
8. MiniBeadbeater DNA isolation from cyanobacteria (and about any bacteria!)
9. Restriction enzyme digestion of DNA
10. Akinete induction of Nostoc punctiforme

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