DISSECTION OF THE FETAL PIG
The period of gestation for pigs is 112‑
115 days and each female may produce a litter of 7‑12 . as development
proceeds, the pig embryos get longer, so an approximate age can be calculated
from the length. (from Odlaug: Laboratory Anatomy of the Fetal Pig. Wm.
C. Brown,publ 1955.
11 mm 21 days
17 mm 35 days
2.8 cm 49 days
4.0 cm 56 days
22 cm 100 days
30 cm birth
COMPARED TO HUMAN LENGTH: from Blechschmidt: The Beginnings of Human Life.
Springer Verlag,1977.
1.6 mm 21 days
4‑6 mm 28 days
7‑12mm 37 days from 4‑6 weeks, the embryos
grow 22‑24mm 52 days about 1 mm per day (crown‑rump), 7 cm 3 lunar months and for
the rest of gestation 12
cm 4 1‑1.5 mm a day except in the 17 cm 5 fourth
month, it is 2 mm/day. 21
cm 6 The increase in weight is six 24.5 7 billion times.
28.5 8
32 9
33.5 10
We have collections of about ten
different sizes of pig embryos and fetuses and many human embryos and fetuses.
EXTERNAL APPEARANCE
There are two measurements used to arrive at these numbers and the age
of the embryo or fetus: crown‑rump length and the degree of cervical
flexion. Then the crown‑heel length is the one used for older fetuses in
humans and at the time of birth ( but crown‑rump was used in the chart
above.)
In study of each embryo, fill in the table on the attached sheet, answering the
questions about each embryo. First measure the crown‑rump length, then
the angle of cervical flexion, using a compass, provided by the
instructor.
Next look at the appearance of the
obvious structures:1. ears, are the external ears present? Is there a shape
change in the older pigs? 2. eyes, are there eyelids, are they closed, and are
the nictitating membranes present? 3. nostrils, are they complete and separate
from the upper lip? 4. mouth, are the lips completed? see your lab book about
development of the human face, palate, 5. limbs, are the joints at the elbows,
wrists, ankles, knees present and are the digits and hooves present? 6. tail,
describe the shape and stiffness, 7. muscle masses, are there obvious somites
or segments down the back? Are there ribs in the thorax or upper trunk? 8.
genitalia, is it male or female? males have scrotal sacs under the tail and
urigenital (UG) openings near the umbilical cord whereas females have UG
openings right underneath the anus (right under the tail) with a genital
papilla in it, 9. hair, distribution of follicles, 10. mammary glands, both
sexes have them along the abdomen, in rows of 5‑7, 11. umbilical cord, in
cross section you can see two large thick walled arteries which carry
unoxygenated blood to the
placenta and one vein which carries
oxygenated blood to the fetal heart from the placenta.
Examine the preparation of the fetus with
fetal membranes: measure the length of the whole placenta. Can you see the
folds on its surface which interdigitate with folds of the uterus)? There are
no villi on pig chorion which correspond to those on the disc of the human
placenta. The connection with the mother is much less intimate, so there is
little bleeding at birth of pigs. In order to see these folds, use the
dissection microscope. Examine the fetal pig in the jar which has an injected
placenta, with arteries in red and veins in blue. Compare it to placentas of
sheep, cat, other mammals shown mounted in jars and to the human placentas we
have seen.
Next, cut open the fetal membranes (the
chorion vesicle is outermost) carefully so you can keep them, BEING CAREFUL NOT
TO CUT A SECOND MEMBRANE INSIDE, then examine the connection of the umbilical
cord with the membranes and see if you can tell the difference between the
allantois, (which is fused with the chorion and is used to store urine in pig
development, and to bring the circulation to the uterine lining to get oxygen
and release wastes) and the amnion, which contains the fluid which protects the
embryo from drying and mechanical injury,
and the outer chorion, and yolk sac.
Look at the slide of human placenta
villi. The round or irregularly shaped structures are sections of the villi.
Each villus after the fourth month has: 1. a blood vessel in it which will
probably contain blood cells. 2. Around the blood vessel is connective tissue
which contains a few cells and a lot of extracellular material which is fibers
and matrix material. 3. Around the connective tissue is the syncytiotrophoblast
(syntrophoblast) which separates the mother's blood from the rest of the
villus, since the mother's blood is in the 4. intervillous space or lacuna
between the villi. MAKE A DRAWING LABELING THESE FOUR STRUCTURES.
FETAL PIG COMPARISON OF EXTERNAL
ANATOMY
NAME‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑
FILL IN THE BLANKS IN THE FOLLOWING TABLE
AFTER EXAMINING THE FETAL PIGS
PIG 1 PIG2 PIG3
PIG4 PIG5 PIG6
PIG7 PIG8 LENGTH ANGLE
OF
CERV. FLEXION NOSTRILS
EYES
LIDS
EARS
LIMBS
KNEES
ANKLES
DIGITS
HOOFS
SOMITES
TAIL
MAMMARY
GENITALS
PAPILLA
SCROTAL SAC
UG OPENING
HAIR
WHAT IS DIFFERENT ABOUT THE PIG PLACENTA
AS COMPARED TO HUMAN, CAT, OTHER MAMMALS?
WHAT IS DIFFERENT ABOUT THE CHORION, ALLANTOIS, YOLK SAC, AND AMNION?
HOW CAN YOU TELL THEM APART? WHAT IS THE RELATIONSHIP OF THE UMBILICAL CORD TO
THEM?
DRAW THE HUMAN PLACENTA SECTION, SHOWING VILLI AND RELATED STRUCTURES.