New List Cell Physiology Lab 2005 Mary Lee Sparling This is a list of possible experiments. We may change the order according to availability of material, or we may decide to do individual projects in place of some of these. Grading will be subjective on participation and lab books turned in. Lab is worth 10 points per lab, for a total of 280. 1 missed lab, made up, no deduction. 2 missed lab, no makeup, get none of the 20 points, 3 missed labs, no makeup, another deduction, etc. Lab data cannot be shared with absentees. Lab books or reports must be done by each individual. If alike between partners, get only half credit.

Jan         31          PROTEIN TEST-PREPARE STANDARD CURVES FOR USE ALL SEMESTER

 Feb         2         Biorad-protein test

             7          PHOSPHATE TEST

             9         ELECTROPHORESIS and detergent and phospholipase  prelab

            14        FERTILIZATION OF SEA URCHIN EGGS AND DRUGS WHICH ARTIFICIALLY ACTIVATE OR PREVENT FERTILIZATION, extract and freeze some eggs for next 3 times

             16 MEMBRANE PHOSPHOLIPASES AS DETECTED BY TLC - sea urchin eggs

            21     extraction of egg pellets MEMBRANE Preparation, use of detergents, prepare lower gels

            23      ELECTROPHORESIS membrane proteins

            28       ELECTROPHORESIS STAIN AND INTERPRETATION

            30       Image J

March   2       WORK ON LAB BOOKS

            7       Stomata       turn in lab books,

            9        Stomata       

            14       Protopl;asmic streaming   CELL MOTILITY;SLIDING FILAMENTS: ELODEA

            16       Pollen germination      

            28       cell division sea urchins DRUGS WHICH EFFECT DIVISION          

            30        CILIA AND FLAGELLA, motors and microtubules FROG MOUTH CILIA

april       4       ACETYLCHOLINESTERASE,

            6 starfish egg maturation MEIOSIS BLOCKS IN EGGS AND THEIR RELEASE TO COMPLETE MEIOSIS ;starfish, 1-methyladenine

            11       PURIFICATION OF FISH MYOSIN AND ACTIN.

            13       MYOSIN ATPASE          

            18      tissue culture chick cartilage and muscle 

            20        PURIFICATION OF TUBULIN FROM 18 DAY CHICK BRAIN- TURN IN LAB BOOKS

            25 assembly actin, tubulin

            27        ANTIGEN-ANTIBODY INTERACTION- OUCHTERLONY AND ELISA

  May   2         antibody stain of our tissue cultures

            4        electrophoresis of myosin, actin, tubulin

            9        REGENERATION PLANERIA, EFFECT OF RETINOIC ACID, DRUGS;

            11       SECRETION, ENDOCYTOSIS           

            16       

 last day-CLEAN UP LAB AND FINISH LAB BOOKS Prepare poster of best experiments.